Educational Resources

ISHSR has taken the initiative to collect digital material that can be used by its members under specific conditions. Often, when we visit scientific meetings, we see excellent illustrations used in lectures of colleagues. In some cases, this colleague will be willing to generously provide you with a copy of the desired image. Or, a protocol may be discussed and shared.  Based on this type of personal exchange of material, ISHSR has decided to offer a place of exchange of digital material on its website. Here, colleagues who want to exchange a part of their scientific material, find the means to enrich their collections of illustrative material, gain access to scientific protocols, and view relevant videos.


IMAGE ARCHIVE (Members Only*)
The photographic images included in the image archive are pixel-defined, with a minimum of XGA (1024 x 768) and a maximum as HD images (1920 x 1080 wide screen). Commercial use and multi-copy reproduction of this material is strictly prohibited. Functions in this area are supposed to be covered by commercial publishers.  A sample image is included below:

home-sample-image.jpg

Current sections within the Image Archive include*:
•  Drawing and Artwork (Endothelial Massage)
•  Light Microscopy (Rat Liver Lobule)
•  Lineage Tracing (Including differentiation of mesothelial cells, etc)

•  Perspectives (History and Morphology of Hepatic Stellate Cells, by Kenjiro Wake, M.D., Ph.D.)
•  Professor Sam French’s EM Images of HSC in Liver Diseases
•  Scanning Electron Microscopy (Human and Rat Fenestrae, Rat Liver Lobule, Rabbit Sinusoid, etc)
•  Transmission Electron Microscopy (Rat Sinusoid)

(* Clicking any of the links above will lead to a login screen if you are not already logged into the website as an ISHSR Member.  The Image Archives are available to Members only).

 


 VIDEO ARCHIVE (Members Only*)
The videos in this section are linked to a special Youtube page that is not available to the public.  Consequently, a very wide variety of formats are included (.MOV, .MPEG4, MP4, .AVI, .WMV, .MPEGPS, .FLV, 3GPP, WebM).  Commercial use and multi-copy reproduction of this material is strictly prohibited. Functions in this area are supposed to be covered by commercial publishers. 

Current content included within the Video Archive*:
•  In Vivo Microscopy of Hepatic Sinusoids

(* Clicking the link above will lead to a login screen if you are not already logged into the website as an ISHSR Member.  The Video Archives are available to Members only).

 


 PROTOCOL BANK (Members Only*)
This section hosts protocols commonly used by investigators involved in Hepatic Sinusoid Research. The protocols are in PDF format, and can be downloaded from the website.  Commercial use and multi-copy reproduction of this material is strictly prohibited. Functions in this area are supposed to be covered by commercial publishers. 

Current protocols within the Protocol Bank include*:
STAINING PROTOCOLS (link):
•  Sirius Red Staining
•  Picro-Sirius Red Collagen

CELL ISOLATION PROTOCOLS (link):
•  Mouse Kupffer & Stellate Cell Isolation

•  Immunomagentic Isolation of Murine LSEC
•  Preparation of Mouse Liver Kupffer Cells and Liver Sinusoidal Endothelial Cells
•  Isolation and Culture of Primary Human HSC's
•  Pronase-Collagenase Liver Perfusion
•  Rat Liver Sinusoidal Endothelial Cell Isolation
•  In Vivo Tracking of Labeled Monocytes

CULTURE MODEL PROTOCOLS (link):
•  Cultivation of Hepatic Stellate Cells in 3-D ECM

•  Necleofection in Primary Cultures of Rat Kupffer Cells

ANALYTICAL PROTOCOLS (link):
•  Analysis of Proteins Expressed in Hepatic Sinusoidal Cells by 2-D SDS Page
•  4-Hydroxyproline Assay

PROTOCOLS RELATED TO SAMPLE PROCESSING FOR IMAGING (link):
•  SEM of Liver SEC
•  SEM of Rat Liver

ANIMAL MODEL PROTOCOLS (link):
•  Coming soon!

(* Clicking any of the links above will lead to a login screen if you are not already logged into the website as an ISHSR Member.  The protocols in the Protocol Bank are available to Members only).